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JoVE Journal Neuroscience
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices
 

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Article DOI: 10.3791/56387-v • 07:32 min • October 12th, 2017
October 12th, 2017 •
*1,2, *1,2, 2, 1,3,4,5
1School of Psychology, South China Normal University, 2School of Life Sciences, South China Normal University, 3Brain Science Institute, South China Normal University, 4Center for Studies of Psychological Application, South China Normal University, 5Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University
* These authors contributed equally

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We describe the puff technique, by which pharmacological reagents can be administered during whole-cell patch-clamp recording, and highlight various aspects of the features that are crucial for its success.

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Puff Technique Pharmacological Administration Whole-cell Patch-clamp Recording GABA Brain Slices Drug Concentration Membrane Receptors Desensitization Acute Prefrontal Cortex Slice Puff Micropipette Frozen Cutting Solution Dissected Head Beaker Ice-cold Cutting Solution Fine Scissors Lateral Skull Portions Fine Spatula Nine-centimeter Petri Dish Cerebellum Olfactory Bulb Cyanoacrylate Glue Vibratome Specimen Holder
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