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İnsan serebrospinal sıvı Endopeptidomic analiz için numune hazırlama
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JoVE Journal 神経科学
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
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İnsan serebrospinal sıvı Endopeptidomic analiz için numune hazırlama

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10:23 min

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December 04, 2017

DOI:

10.3791/56244-v

DOI:
10.3791/56244-v

•

10:23 min
December 04, 2017

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2 Views
, , , , , , ,
1Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry,Sahlgrenska Academy at University of Gothenburg, 2Clinical Neurochemistry Laboratory,Sahlgrenska University Hospital, 3Department of Molecular Neuroscience,UCL Institute of Neurology

概要

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Endojen peptidler insan beyin-omurilik sıvısı (bos) kitle spektrometrik analizi için bir yöntem sunulur. Moleküler ağırlık kesme filtrasyon, Kromatografik ön ayırma, kitle spektrometrik analizi ve peptid kimlik stratejileri sonraki bir arada istihdam ederek, yaklaşık on göre bilinen CSF peptidome genişletmek olası Önceki çalışmalar için.

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Cite this Article

Hansson, K. T., Skillbäck, T., Pernevik, E., Holmén-Larsson, J., Brinkmalm, G., Blennow, K., Zetterberg, H., Gobom, J. Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid. J. Vis. Exp. (130), e56244, doi:10.3791/56244 (2017).

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