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Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
JoVE Journal
がん研究
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JoVE Journal がん研究
Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

11,794 Views

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10:51 min

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August 23, 2017

DOI:

10.3791/56081-v

DOI:
10.3791/56081-v

•

10:51 min
August 23, 2017

•

8 Views
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1Molecular Medicine Division,Translational Genomics Research Institute, 2Agilent Technologies

概要

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Here, a method is described for the preparation of 3-dimensional (3D) spheroid co-culture of pancreatic cancer cells and fibroblasts, followed by measurement of metabolic functions using an extracellular flux analyzer.

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Cite this Article

Noel, P., Muñoz, R., Rogers, G. W., Neilson, A., Von Hoff, D. D., Han, H. Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts. J. Vis. Exp. (126), e56081, doi:10.3791/56081 (2017).

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