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体外在拟南芥中受精卵分化和胚模式的活体细胞成像的胚珠培养
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JoVE Journal 発生生物学
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
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体外在拟南芥中受精卵分化和胚模式的活体细胞成像的胚珠培养

10,720 Views

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05:41 min

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September 11, 2017

DOI:

10.3791/55975-v

DOI:
10.3791/55975-v

•

05:41 min
September 11, 2017

•

32 Views
, , ,
1Division of Biological Science, Graduate School of Science,Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO,Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM),Nagoya University

概要

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这份手稿描述了一个体外胚珠培养方法, 使受精卵和胚胎的活细胞成像的拟南芥。该方法用于在受精卵分化过程中对细胞内动力学进行可视化, 并对发育中的胚胎的胞命运规范进行研究。

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Cite this Article

Kurihara, D., Kimata, Y., Higashiyama, T., Ueda, M. In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana. J. Vis. Exp. (127), e55975, doi:10.3791/55975 (2017).

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