Dergi
/
Gelişim Biyolojisi
/
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

10,720 Views

•

05:41 min

•

September 11, 2017

DOI:

10.3791/55975-v

DOI:
10.3791/55975-v

•

05:41 min
September 11, 2017

•

32 Views
, , ,
1Division of Biological Science, Graduate School of Science,Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO,Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM),Nagoya University

Özet

Automatically generated

This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

Explore More Videos

In Vitro Ovule CultivationLive-cell ImagingZygote PolarizationEmbryo PatterningArabidopsis ThalianaPDMS Micropillar ArrayN5T MediumSilique DissectionOvule TransferCover Glass

Read Article

Cite this Article

Kurihara, D., Kimata, Y., Higashiyama, T., Ueda, M. In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana. J. Vis. Exp. (127), e55975, doi:10.3791/55975 (2017).

Download .ris file

Copy

Share Video

.

Copy