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In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
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JoVE 杂志 发育生物学
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

DOI:
10.3791/55975-v

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05:41 min

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September 11, 2017

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, , ,
1Division of Biological Science, Graduate School of Science,Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO,Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM),Nagoya University

Chapters

  • 00:05Title
  • 00:45Poly(Dimethylsoloxane) Micropillar Array Divide Preparation
  • 02:03Ovule Extraction and Time-lapse Imaging
  • 04:14Results: Representative Live-imaging of Zygote Polarization and Embryo Patterning
  • 05:03Conclusion

Summary

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This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

Tags

In Vitro Ovule CultivationLive-cell ImagingZygote PolarizationEmbryo PatterningArabidopsis ThalianaPDMS Micropillar ArrayN5T MediumSilique DissectionOvule TransferCover Glass

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