Journal
/
发育生物学
/
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

10,720 Views

•

05:41 min

•

September 11, 2017

DOI:

10.3791/55975-v

DOI:
10.3791/55975-v

•

05:41 min
September 11, 2017

•

32 Views
, , ,
1Division of Biological Science, Graduate School of Science,Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO,Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM),Nagoya University

Summary

Automatically generated

This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

Explore More Videos

In Vitro Ovule CultivationLive-cell ImagingZygote PolarizationEmbryo PatterningArabidopsis ThalianaPDMS Micropillar ArrayN5T MediumSilique DissectionOvule TransferCover Glass

Read Article

Cite this Article

Kurihara, D., Kimata, Y., Higashiyama, T., Ueda, M. In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana. J. Vis. Exp. (127), e55975, doi:10.3791/55975 (2017).

Download .ris file

Copy

Share Video

.

Copy