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Long-term Live Imaging of Drosophila Eye Disc
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Long-term Live Imaging of Drosophila Eye Disc

Long-term Live Imaging of Drosophila Eye Disc

DOI:
10.3791/55748-v

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06:08 min

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May 06, 2017

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, ,
1Institute of Genomic Sciences,National Yang-Ming University, 2Institute of Molecular Biology,Academia Sinica

章

  • 00:05標題
  • 01:04Disc Dissection
  • 02:43Mounting
  • 04:04Confocal Microscopy
  • 04:48Results: Sustained Photoreceptor Differentiation during 10 h Live Imaging
  • 05:44Conclusion

概要

自動翻訳

自動翻訳

This protocol describes a detailed method for the long-term ex vivo culture and live imaging of a Drosophila imaginal disc. It demonstrates photoreceptor differentiation and ommatidial rotation within the 10 h period of live imaging of the eye disc. The protocol is simple and does not require expensive setup.

タグ

Live ImagingDrosophilaEye DiscImaginal DiscCell ProliferationCell DifferentiationApoptosisCell Competition発生生物学Cell MorphologyCell MigrationDissectionMountingCulture MediumPBSForcepsScissorsMouth HookBrainOptical StalkCoverslipO-ringAgaroseChamber

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