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Biologie du développement
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Long-term Live Imaging of Drosophila Eye Disc
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Long-term Live Imaging of Drosophila Eye Disc

Long-term Live Imaging of Drosophila Eye Disc

DOI:
10.3791/55748-v

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06:08 min

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May 06, 2017

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, ,
1Institute of Genomic Sciences,National Yang-Ming University, 2Institute of Molecular Biology,Academia Sinica

Chapitres

  • 00:05Titre
  • 01:04Disc Dissection
  • 02:43Mounting
  • 04:04Confocal Microscopy
  • 04:48Results: Sustained Photoreceptor Differentiation during 10 h Live Imaging
  • 05:44Conclusion

Summary

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Traduction automatique

This protocol describes a detailed method for the long-term ex vivo culture and live imaging of a Drosophila imaginal disc. It demonstrates photoreceptor differentiation and ommatidial rotation within the 10 h period of live imaging of the eye disc. The protocol is simple and does not require expensive setup.

Tags

Live ImagingDrosophilaEye DiscImaginal DiscCell ProliferationCell DifferentiationApoptosisCell CompetitionBiologie du développementCell MorphologyCell MigrationDissectionMountingCulture MediumPBSForcepsScissorsMouth HookBrainOptical StalkCoverslipO-ringAgaroseChamber

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