このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
9,734 Views
•
13:10 min
•
June 09, 2017
DOI:
概要
Automatically generated
Here, it is demonstrated how the in situ Proximity Ligation Assay (PLA) can be used to detect and visualize the direct protein-protein interactions between ATM and p53 in suspension cell cultures exposed to genotoxic stress.
Read Article
Cite this Article
Bahjat, M., Bloedjes, T. A., van der Veen, A., de Wilde, G., Maas, C., Guikema, J. E. J. Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay. J. Vis. Exp. (124), e55703, doi:10.3791/55703 (2017).
Copy