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Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

9,748 Views

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13:10 min

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June 09, 2017

DOI:

10.3791/55703-v

DOI:
10.3791/55703-v

•

13:10 min
June 09, 2017

•

10 Views
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1Patoloji Bölümü,Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

Özet

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Here, it is demonstrated how the in situ Proximity Ligation Assay (PLA) can be used to detect and visualize the direct protein-protein interactions between ATM and p53 in suspension cell cultures exposed to genotoxic stress.

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Cite this Article

Bahjat, M., Bloedjes, T. A., van der Veen, A., de Wilde, G., Maas, C., Guikema, J. E. J. Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay. J. Vis. Exp. (124), e55703, doi:10.3791/55703 (2017).

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