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Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
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JoVE Journal 遺伝学
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

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08:56 min

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June 27, 2017

DOI:

10.3791/55505-v

DOI:
10.3791/55505-v

•

08:56 min
June 27, 2017

•

2 Views
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1Division of Nephrology, First Department of Integrated Medicine,Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University

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Here we present a protocol for the detection of microRNA expression in rat peritoneal membrane using quantitative real-time reverse-transcription polymerase chain reaction. This method is suitable for studying the microRNA expression profile in rat peritoneal membrane in several pathological conditions.

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Keyword Extraction:MiRNAPeritoneal MembraneQuantitative Real-time PCRRNA PurificationRatHomogenizationPhenol-guanidineChloroformEthanolSpin Column

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Cite this Article

Hirai, K., Yoshizawa, H., Imai, T., Igarashi, Y., Hirahara, I., Ookawara, S., Ishibashi, K., Morishita, Y. Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR. J. Vis. Exp. (124), e55505, doi:10.3791/55505 (2017).

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