Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Detection of microRNA Expression in Peritoneal…
JoVE Journal
Genetics

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Genetics
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
 

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Article DOI: 10.3791/55505-v • 08:56 min • June 27th, 2017
June 27th, 2017 •
1, 2, 2, 2, 2, 2, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

Capitoli

Riepilogo
Automatic Translation

Here we present a protocol for the detection of microRNA expression in rat peritoneal membrane using quantitative real-time reverse-transcription polymerase chain reaction. This method is suitable for studying the microRNA expression profile in rat peritoneal membrane in several pathological conditions.

Tags

MicroRNA Expression Peritoneal Membrane Rats Quantitative Real-time PCR Purification Detection Inhalational Anesthesia 4% Isoflurane Abdominal Skin 70% Ethanol Cork Sheet Longitudinal Incision Scissors Forceps Horizontal Incisions Lowest Rib Bone Diaphragm Lateral Region Surgical Scissors PPS Petri Dish 20 Milligrams Peritoneal Membrane Sample Glass Homogenizer Phenol Guanidine Based Lysis Reagent Ice Pestle Homogenization Bio-polymer Shredding System Micro Centrifuge Spin Column
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter