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맞춤형 HPLC 정화 프로토콜 즉, 올리고머 형성 할 수있는 수율 고순도 아밀로이드 베타 42과 아밀로이드 베타 펩티드 (40),
章
- 00:05標題
- 00:34Preparative HPLC Purification of the Aβ40 or Aβ42 Peptide
- 02:17Purification of Aβ Peptide
- 03:26Analytical HPLC Analysis of the Purified Aβ Protein
- 04:42Results: HPLC Measurements of Purified Aβ42 Peptide
- 05:53Conclusion
Herein we report a tailored HPLC purification protocol that yields high-purity amyloid beta 42 (Aβ42) and amyloid beta 40 (Aβ40) peptides, capable of oligomer formation. Amyloid beta is a highly aggregation prone, hydrophobic peptide implicated in Alzheimer's disease. The amyloidogenic nature of the peptide makes its purification a challenge.
