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Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
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Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

8,129 Views

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07:41 min

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October 02, 2017

DOI:

10.3791/55369-v

DOI:
10.3791/55369-v

•

07:41 min
October 02, 2017

•

2 Views
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1Department of Medicinal Chemistry and Molecular Pharmacology,Purdue University

概要

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Sequential salt extraction of chromatin bound proteins is a useful tool for determining the binding properties of large protein complexes. This method can be employed to evaluate the role of individual subunits or domains in the overall affinity of a protein complex to bulk chromatin.

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Cite this Article

Porter, E. G., Connelly, K. E., Dykhuizen, E. C. Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes. J. Vis. Exp. (128), e55369, doi:10.3791/55369 (2017).

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