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Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
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Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

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07:41 min

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October 02, 2017

DOI:

10.3791/55369-v

DOI:
10.3791/55369-v

•

07:41 min
October 02, 2017

•

2 Views
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1Department of Medicinal Chemistry and Molecular Pharmacology,Purdue University

Summary

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Sequential salt extraction of chromatin bound proteins is a useful tool for determining the binding properties of large protein complexes. This method can be employed to evaluate the role of individual subunits or domains in the overall affinity of a protein complex to bulk chromatin.

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Porter, E. G., Connelly, K. E., Dykhuizen, E. C. Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes. J. Vis. Exp. (128), e55369, doi:10.3791/55369 (2017).

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