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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
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JoVE Journal 発生生物学
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

8,407 Views

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08:21 min

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March 16, 2017

DOI:

10.3791/55268-v

DOI:
10.3791/55268-v

•

08:21 min
March 16, 2017

•

2 Views
,
1Department of Biomedical Sciences, School of Veterinary Medicine,University of Pennsylvania

概要

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Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.

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Human Pluripotent Stem CellsTrophoblastic CellsBone Morphogenic 4Nodal Signaling PathwaysPlacental FormationExtracellular MatrixMouse Embryonic Fibroblast-conditioned MediumBFGFDispaseDifferentiation Medium

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Cite this Article

Wang, J., Anguera, M. C. In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells. J. Vis. Exp. (121), e55268, doi:10.3791/55268 (2017).

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