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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
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March 16, 2017
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Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.
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Wang, J., Anguera, M. C. In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells. J. Vis. Exp. (121), e55268, doi:10.3791/55268 (2017).
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