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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

DOI:
10.3791/55268-v

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08:21 min

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March 16, 2017

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1Department of Biomedical Sciences, School of Veterinary Medicine,University of Pennsylvania

Capitoli

  • 00:05Titolo
  • 00:49Transitioning hESCs to Feeder-free Conditions
  • 02:59hESC Differentiation Using BMP4/A/P
  • 04:02Trophoblastic Cell Transfection Preparation
  • 04:41Transfection Using siRNAs
  • 06:33Results: Rapid Differentiation for Visualization and Analysis
  • 07:51Conclusion

Summary

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Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.

Tags

Human Pluripotent Stem CellsTrophoblastic CellsBone Morphogenic 4Nodal Signaling PathwaysPlacental FormationExtracellular MatrixMouse Embryonic Fibroblast-conditioned MediumBFGFDispaseDifferentiation Medium

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