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An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
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February 09, 2017
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Angiogenesis involves multi-cell, multi-system interactions that need to be investigated in a physiologically relevant environment. The objective of this study is to demonstrate the ability of the rat mesentery culture model to make time-lapse comparisons of intact microvascular networks during angiogenesis.
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Ex VivoTime-lapse ImagingRat Mesenteric Microvascular NetworksMicrovascular RemodelingEndothelial Cell InteractionsBlood Lymphatic Endothelial Cell ConnectionsAnti-angiogenic Drug EffectsPlexiglass PlatformAnesthetized Adult Male RatAbdominal IncisionMesenteric WindowsSterile SalinePBSCell Culture PlatePolycarbonate Filter Membrane
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Azimi, M. S., Motherwell, J. M., Murfee, W. L. An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks. J. Vis. Exp. (120), e55183, doi:10.3791/55183 (2017).
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