需要订阅 JoVE 才能查看此. 登录或开始免费试用。
An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
6,740 Views
•
06:54 min
•
February 09, 2017
DOI:
Summary
Automatically generated
Angiogenesis involves multi-cell, multi-system interactions that need to be investigated in a physiologically relevant environment. The objective of this study is to demonstrate the ability of the rat mesentery culture model to make time-lapse comparisons of intact microvascular networks during angiogenesis.
Explore More Videos
Ex VivoTime-lapse ImagingRat Mesenteric Microvascular NetworksMicrovascular RemodelingEndothelial Cell InteractionsBlood Lymphatic Endothelial Cell ConnectionsAnti-angiogenic Drug EffectsPlexiglass PlatformAnesthetized Adult Male RatAbdominal IncisionMesenteric WindowsSterile SalinePBSCell Culture PlatePolycarbonate Filter Membrane
Read Article
Cite this Article
Azimi, M. S., Motherwell, J. M., Murfee, W. L. An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks. J. Vis. Exp. (120), e55183, doi:10.3791/55183 (2017).
Copy