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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
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JoVE Journal 免疫学と感染
Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

DOI:
10.3791/55087-v

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10:39 min

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March 10, 2017

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, , , , , ,
1Division of Biomedical Science, Faculty of Medicine,University of Tsukuba, 2Department of Basic Biomedical Science,Universidad Europea de Madrid, 3Human Biology Program, School of Integrative and Global Majors,University of Tsukuba, 4Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa,Instituto de Salud Carlos III, 5Division of Microbiology, Department of Medicine, School of Medicine,Universidad Complutense, 6Biology of Gram-Positive Pathogens, Department of Microbiology,Institut Pasteur, Paris, France, 7ERL3526,CNRS, Paris, France

章

  • 00:05標題
  • 00:51Conjugation Using the Filter-mating Method
  • 03:10Phage Amplification on the Donor
  • 04:43Measuring the Phage Titer
  • 06:01Phage Transduction
  • 07:54Transformation Assay
  • 09:41Results: Successful Horizontal Gene Transfer
  • 10:10Conclusion

概要

自動翻訳

自動翻訳

We describe here three different protocols for the in vitro investigation of conjugation, transduction, and natural transformation in Staphylococcus aureus.

タグ

Horizontal Gene TransferStaphylococcus AureusConjugationTransductionNatural TransformationAntibiotic ResistanceCFR GeneDonorRecipientFilter MembraneSelective AgarColony PCRAntibiogramMICDisc Diffusion

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