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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
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March 10, 2017
DOI:
1Division of Biomedical Science, Faculty of Medicine,University of Tsukuba, 2Department of Basic Biomedical Science,Universidad Europea de Madrid, 3Human Biology Program, School of Integrative and Global Majors,University of Tsukuba, 4Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa,Instituto de Salud Carlos III, 5Division of Microbiology, Department of Medicine, School of Medicine,Universidad Complutense, 6Biology of Gram-Positive Pathogens, Department of Microbiology,Institut Pasteur, Paris, France, 7ERL3526,CNRS, Paris, France
Summary
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We describe here three different protocols for the in vitro investigation of conjugation, transduction, and natural transformation in Staphylococcus aureus.
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Cafini, F., Thi Le Thuy, N., Román, F., Prieto, J., Dubrac, S., Msadek, T., Morikawa, K. Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus. J. Vis. Exp. (121), e55087, doi:10.3791/55087 (2017).
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