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리파제 및 포스 후보자에 대한 기질 특이성을 정의
JoVE Journal
生化学
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JoVE Journal 生化学
Defining Substrate Specificities for Lipase and Phospholipase Candidates

리파제 및 포스 후보자에 대한 기질 특이성을 정의

DOI:

08:59 min

November 23, 2016

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  • 00:05標題
  • 01:02Use Artificial Substrates for Optimizing Enzyme Activities of (Phospho)lipases
  • 02:20In Vivo Identification of the Physiological Substrate of a Lipase
  • 05:48In Vitro Identification of the Physiological Substrate of Diacylglycerol (DAG) Lipase
  • 07:00Results: Predicted Phospholipase SMc01003 Acts on Artificial p-Nitrophenyl Acyl Ester and DAG
  • 08:01Conclusion

概要

自動翻訳

Many predicted (phospho)lipases are poorly characterized with regard to their substrate specificities and physiological functions. Here we provide a protocol to optimize enzyme activities, search for natural substrates, and propose physiological functions for these enzymes.

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