This content is Free Access.
STAT3スプライスバリアント転写産物を定量化するために絶対的および相対的定量PCRのデータをマージ
14,549 Views
•
11:19 min
•
October 09, 2016
DOI:
概要
Automatically generated
Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.
Read Article
Cite this Article
Turton, K. B., Esnault, S., Delain, L. P., Mosher, D. F. Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts. J. Vis. Exp. (116), e54473, doi:10.3791/54473 (2016).
Copy