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Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
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JoVE Journal 遺伝学
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

DOI:
10.3791/54399-v

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08:53 min

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June 10, 2017

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, , , , ,
1Innovation Laboratory, Center for Molecular Oncology,Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility,Weill Cornell Medical College, 3Department of Laboratory Medicine,University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine,Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine,Weill Cornell Medical College, 6Department of Laboratory Medicine,Memorial Sloan Kettering Cancer Center

章

  • 00:05標題
  • 01:19Cell Suspension Preparation for Sorting
  • 03:00Compensation Control Setup
  • 04:53Hodgkin Reed-Sternberg-, B-, and T-cell Isolation
  • 06:07Results: Representative DNA Library and Copy Number Gains and Losses Analyses
  • 08:02Conclusion

概要

自動翻訳

自動翻訳

Here, we describe a combined flow cytometric cell sorting and low-input, next-generation library construction protocol designed to produce high-quality, whole-exome data from the Hodgkin Reed-Sternberg (HRS) cells of classical Hodgkin lymphoma (CHL).

タグ

Flow-sortingExome SequencingReed-Sternberg CellsClassical Hodgkin LymphomaCHLHRS CellsNext-generation SequencingTumor-derived DNADiagnosisClassificationPrognosisTherapyFlow CytometryAntibody CocktailThawing MediumSorting Medium

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