ジャーナル
/
化学
/
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
JoVE Journal
化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 化学
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

37,072 Views

•

09:14 min

•

July 20, 2016

DOI:

10.3791/54157-v

DOI:
10.3791/54157-v

•

09:14 min
July 20, 2016

•

56 Views
,
1School of Chemistry, The Australian Centre for Nanomedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,The University of New South Wales

概要

Automatically generated

This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.

Explore More Videos

Protein BioconjugatesCysteine-maleimide ChemistrySite-specific ModificationAntibody Drug ConjugatesNano-medicineFluorescent MicroscopySystems ChemistryCytochrome CFPLCRuthenium Maleimide ComplexTCEP

Read Article

Cite this Article

Mason, A. F., Thordarson, P. Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry. J. Vis. Exp. (113), e54157, doi:10.3791/54157 (2016).

Download .ris file

Copy

Share Video

.

Copy