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Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
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Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

37,072 Views

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09:14 min

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July 20, 2016

DOI:

10.3791/54157-v

DOI:
10.3791/54157-v

•

09:14 min
July 20, 2016

•

56 Views
,
1School of Chemistry, The Australian Centre for Nanomedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,The University of New South Wales

Summary

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This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.

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Cite this Article

Mason, A. F., Thordarson, P. Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry. J. Vis. Exp. (113), e54157, doi:10.3791/54157 (2016).

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