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Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

DOI:
10.3791/53936-v

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07:17 min

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April 14, 2016

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, ,
1Department of Urology,Johns Hopkins University

章

  • 00:05標題
  • 00:41Hind Limb Long Bone Dissection
  • 03:01Long Bone Preparation For Bone Marrow Isolation
  • 04:25Bone Marrow Isolation
  • 05:29Results: Representative Downstream Analyses and Applications of Rapid DIssected Mouse Long Bones and Bone Marrow
  • 06:28Conclusion

概要

自動翻訳

自動翻訳

Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

タグ

MurineHind LimbLong Bone DissectionBone Marrow IsolationBone BiologyCancer Metastasis免疫学FemurTibiaAnatomyDissectionIsolationDownstream Analysis

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