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Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

DOI:
10.3791/53936-v

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07:17 min

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April 14, 2016

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, ,
1Department of Urology,Johns Hopkins University

Chapters

  • 00:05Title
  • 00:41Hind Limb Long Bone Dissection
  • 03:01Long Bone Preparation For Bone Marrow Isolation
  • 04:25Bone Marrow Isolation
  • 05:29Results: Representative Downstream Analyses and Applications of Rapid DIssected Mouse Long Bones and Bone Marrow
  • 06:28Conclusion

Summary

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Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

Tags

Keywords: MurineHind LimbLong Bone DissectionBone Marrow IsolationBone BiologyCancer MetastasisImmunologyFemurTibiaAnatomyDissectionIsolationDownstream Analysis

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