このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
11,752 Views
•
10:13 min
•
February 10, 2016
DOI:
概要
Automatically generated
Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal cultures.
Read Article
Cite this Article
Li, H., Aksenova, M., Bertrand, S. J., Mactutus, C. F., Booze, R. Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons. J. Vis. Exp. (108), e53697, doi:10.3791/53697 (2016).
Copy