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Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
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JoVE Journal 発生生物学
Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

7,776 Views

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11:13 min

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February 02, 2016

DOI:

10.3791/53474-v

DOI:
10.3791/53474-v

•

11:13 min
February 02, 2016

•

5 Views
1Institut Curie, 2UMR 3387,CNRS, 3PSL Research University, 4Université Paris-Sud

概要

Automatically generated

In Xenopus embryos, cells from the roof of the blastocoel are pluripotent and can be programmed to generate various tissues. Here, we describe protocols to use amphibian blastocoel roof explants as an assay system to investigate key in vivo and in vitro features of early neural development.

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Cite this Article

Durand, B. C. Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo. J. Vis. Exp. (108), e53474, doi:10.3791/53474 (2016).

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