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Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
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JoVE 杂志 发育生物学
Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

DOI:
10.3791/53474-v

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11:13 min

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February 02, 2016

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1Institut Curie, 2UMR 3387,CNRS, 3PSL Research University, 4Université Paris-Sud

Chapters

  • 00:05Title
  • 01:14Xenopus laevis Tadpole Anterior Neutral Tube Flat-mounting
  • 03:05Animal Cap Explant Induction Using Anion Exchange Resin Beads
  • 04:58Animal Cap Dissociation and Reaggregation
  • 06:52Animal Cap Explant Implantation onto Xenopus laevis Embryo Neural Plates
  • 08:12Results: Representative Xenopus Explants
  • 10:13Conclusion

Summary

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In Xenopus embryos, cells from the roof of the blastocoel are pluripotent and can be programmed to generate various tissues. Here, we describe protocols to use amphibian blastocoel roof explants as an assay system to investigate key in vivo and in vitro features of early neural development.

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Keywords: XenopusEmbryonic ExplantsNeural DevelopmentIn VitroIn VivoFate AcquisitionCell MigrationNeural CellsReprogrammingInduced Pluripotent Stem CellsNeural-derived CellsCell SegregationFlat-mountAnterior Neural TubeHybridizationNotochordOtic VesiclesMesodermGlycerolDissectionMicroscopy

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