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Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
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JoVE Journal 発生生物学
Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

DOI:
10.3791/53342-v

•

11:48 min

•

January 17, 2016

•

,
1Division of Animal Sciences, School of Biosciences,University of Nottingham

章

  • 00:05標題
  • 00:48Preparation of Heparin Beads for Grafting
  • 02:58Preparation of AG 1-X2 Beads for Grafting
  • 05:21Preparing Tungsten Needles and Embryos for Bead Grafts
  • 09:24Results: Evaluating Expression of MyoD in Limbs
  • 11:06Conclusion

概要

自動翻訳

自動翻訳

By grafting beads soaked in growth factors or specific inhibitors of signaling pathways into developing embryos it is possible to directly test their effects in vivo. In this protocol beads are grafted into the limb bud to determine the effects of these molecules on gene expression and signal transduction.

タグ

GraftingChicken EmbryoLimb DevelopmentSignal TransductionGene ExpressionGrowth FactorsFGF18Bead ImplantationIn Vivo Assay発生生物学

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