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Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
Chapters
- 00:05Title
- 00:48Preparation of Heparin Beads for Grafting
- 02:58Preparation of AG 1-X2 Beads for Grafting
- 05:21Preparing Tungsten Needles and Embryos for Bead Grafts
- 09:24Results: Evaluating Expression of MyoD in Limbs
- 11:06Conclusion
By grafting beads soaked in growth factors or specific inhibitors of signaling pathways into developing embryos it is possible to directly test their effects in vivo. In this protocol beads are grafted into the limb bud to determine the effects of these molecules on gene expression and signal transduction.
