ジャーナル
/
発生生物学
/
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

DOI:
10.3791/53280-v

•

09:16 min

•

December 14, 2015

•

,
1皮膚学部,UCSD Stem Cell Program, University of California, San Diego, 2Department of Cellular and Molecular Medicine,UCSD Stem Cell Program, University of California, San Diego

章

  • 00:05標題
  • 00:50Preparation of Devitalized Human Dermis
  • 02:21Genetic Modification of Primary Human Keratinocytes
  • 04:33Building an Organotypic Cassette
  • 05:09Organotypic Skin Cultures
  • 07:03Results: Morphology and Gene Expression Analysis of Genetically Modified Human Epidermis
  • 08:28Conclusion

概要

自動翻訳

自動翻訳

The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.

タグ

Organotypic Skin CulturesGenetically Modified KeratinocytesDevitalized Human DermisEpidermal DifferentiationStratificationRetrovirusesGene OverexpressionGene KnockdownSkin HomeostasisGene Expression ProfilingImmunostainingChromatin ImmunoprecipitationHigh-throughput Sequencing

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Using Primary Neurosphere Cultures to Study Primary Cilia

A Modified Trier Social Stress Test for Vulnerable Mexican American Adolescents

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Read Article