ジャーナル
/
発生生物学
/
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

7,440 Views

•

08:14 min

•

December 03, 2015

DOI:

10.3791/52910-v

DOI:
10.3791/52910-v

•

08:14 min
December 03, 2015

•

1 Views
, ,
1Primate Research Center,University of Wisconsin-Madison, 2Department of Pathology and Laboratory Medicine,University of Wisconsin School of Medicine and Public Health

概要

Automatically generated

This protocol describes the efficient induction of hemogenic endothelium and multipotential hematopoietic progenitors from human pluripotent stem cells via the forced expression of transcription factors.

Explore More Videos

Hemogenic EndotheliumHematopoietic CellsPluripotent Stem CellsTranscription FactorsETV2GATA1GATA2TAL1LMO2Endothelial-hematopoietic TransitionErythroidMegakaryocyticIn Vitro Modeling

Read Article

Cite this Article

Elcheva, I., Brok-Volchanskaya, V., Slukvin, I. Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells. J. Vis. Exp. (106), e52910, doi:10.3791/52910 (2015).

Download .ris file

Copy

Share Video

.

Copy