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Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

7,440 Views

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08:14 min

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December 03, 2015

DOI:

10.3791/52910-v

DOI:
10.3791/52910-v

•

08:14 min
December 03, 2015

•

1 Views
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1Primate Research Center,University of Wisconsin-Madison, 2Department of Pathology and Laboratory Medicine,University of Wisconsin School of Medicine and Public Health

Summary

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This protocol describes the efficient induction of hemogenic endothelium and multipotential hematopoietic progenitors from human pluripotent stem cells via the forced expression of transcription factors.

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Cite this Article

Elcheva, I., Brok-Volchanskaya, V., Slukvin, I. Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells. J. Vis. Exp. (106), e52910, doi:10.3791/52910 (2015).

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