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在脑切片检测轴突本地化mRNAs的高空间分辨率<em>原位</em>杂交
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JoVE Journal 神経科学
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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在脑切片检测轴突本地化mRNAs的高空间分辨率<em>原位</em>杂交

11,609 Views

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11:24 min

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June 17, 2015

DOI:

10.3791/52799-v

DOI:
10.3791/52799-v

•

11:24 min
June 17, 2015

•

8 Views
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1College of Physicians and Surgeons,Columbia University

概要

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RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.

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Axonally Localized MRNAsIn Situ HybridizationRNAscopeAxonal CounterstainFluorescence ImmunohistochemistryHistological DyesAtf4 MRNATranscriptome AnalysisVertebrate AxonsAxon PathfindingAxon BranchingNeurodegenerationSingle-molecule DetectionSubcellular LocalizationMature Mouse BrainHuman Brain

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Baleriola, J., Jean, Y., Troy, C., Hengst, U. Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization. J. Vis. Exp. (100), e52799, doi:10.3791/52799 (2015).

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