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Neuroscience

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JoVE Journal Neuroscience
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
 

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Article DOI: 10.3791/52799-v • 11:24 min • June 17th, 2015
June 17th, 2015 •
1, 1, 1, 1
1College of Physicians and Surgeons, Columbia University

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RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.

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Axonally Localized MRNAs In Situ Hybridization Axon Pathfinding Axon Branching Local Translation Transcriptome Analysis Cultured Neurons Whole Tissues In Vivo Functional Relevance Visualization Of MRNAs ISH Technologies Single-molecule Level Ultrasensitive RNA ISH Technology RNAscope ISH Axonal Counterstain Fluorescence Immunohistochemistry Histological Dyes Atf4 MRNA
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