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Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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June 17, 2015
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RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.
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Baleriola, J., Jean, Y., Troy, C., Hengst, U. Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization. J. Vis. Exp. (100), e52799, doi:10.3791/52799 (2015).
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