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ヒト免疫不全ウイルスの複製フィットネスを決定するためのペアごとの成長競合アッセイ
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
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ヒト免疫不全ウイルスの複製フィットネスを決定するためのペアごとの成長競合アッセイ

10,907 Views

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11:19 min

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May 04, 2015

DOI:

10.3791/52610-v

DOI:
10.3791/52610-v

•

11:19 min
May 04, 2015

•

2 Views
, , , , , , , , , , ,
1Department of Microbiology,University of Washington, 2Departments of Medicine and Laboratory Medicine,University of Washington, 3U.S Military HIV Research Program,Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation

概要

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Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

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HIV-1Viral Replication FitnessIn Vitro Fitness AssayGrowth Competition AssayMultiplicity Of Infection (MOI)Viral DetectionRelative Fitness Calculation

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Cite this Article

Manocheewa, S., Lanxon-Cookson, E. C., Liu, Y., Swain, J. V., McClure, J., Rao, U., Maust, B., Deng, W., Sunshine, J. E., Kim, M., Rolland, M., Mullins, J. I. Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses. J. Vis. Exp. (99), e52610, doi:10.3791/52610 (2015).

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