ジャーナル
/
医学
/
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells
JoVE Journal
医学
This content is Free Access.
JoVE Journal 医学
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

13,507 Views

•

11:37 min

•

February 18, 2015

DOI:

10.3791/52446-v

DOI:
10.3791/52446-v

•

11:37 min
February 18, 2015

•

2 Views
, ,
1Women’s Malignancies Branch,National Cancer Institute

概要

Automatically generated

Tumor-initiating cells (TICs) may represent a viable therapeutic target for the treatment of ovarian cancer, a highly recurrent and fatal disease. We present a protocol for culture conditions that enrich for this highly tumorigenic population of cells.

Explore More Videos

Ovarian CancerTumor-initiating CellsTICsIn Vitro EnrichmentSphere FormationTumorigenicityStem Cell MarkersCD133ALDHLow AttachmentSerum-free MediaGrowth FactorsAdherent CellsNon-adherent CellsFloater CellsSignaling PathwaysDrug Targeting

Read Article

Cite this Article

House, C. D., Hernandez, L., Annunziata, C. M. In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells. J. Vis. Exp. (96), e52446, doi:10.3791/52446 (2015).

Download .ris file

Copy

Share Video

.

Copy