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Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

DOI:
10.3791/52381-v

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10:11 min

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September 27, 2014

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, , ,
1Laboratory of Virology, Division of Intramural Research,National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2Research Technology Branch, Division of Intramural Research,National Institute of Allergy and Infectious Diseases, National Institutes of Health

章

  • 00:00標題
  • 03:18Step 1: Initial Production of trVLPs
  • 05:41Step 2: Preparation and Infection of Target Cells
  • 07:13Step 3: Harvest of Cells and Determination of Reporter Activity
  • 08:55結果
  • 09:25Conclusions

概要

自動翻訳

自動翻訳

Work with infectious Ebola viruses is restricted to biosafety level 4 laboratories. Tetracistronic minigenome-containing replication and transcription-competent virus like particles (trVLPs) represent a lifecycle modeling system that allows us to safely model multiple infectious cycles under biosafety level 2 conditions, relying exclusively on Ebola virus components.

タグ

Ebola VirusVirus-like ParticlesTetracistronic MinigenomesBiosafety Level 2Virus Lifecycle ModelingReplication And Transcription-competent Virus-like ParticlesVP40GP12VP24MorphogenesisBuddingEntryGenome ReplicationTranscription

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