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Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

35,699 Views

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10:11 min

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September 27, 2014

DOI:

10.3791/52381-v

DOI:
10.3791/52381-v

•

10:11 min
September 27, 2014

•

11 Views
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1Laboratory of Virology, Division of Intramural Research,National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2Research Technology Branch, Division of Intramural Research,National Institute of Allergy and Infectious Diseases, National Institutes of Health

Summary

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Work with infectious Ebola viruses is restricted to biosafety level 4 laboratories. Tetracistronic minigenome-containing replication and transcription-competent virus like particles (trVLPs) represent a lifecycle modeling system that allows us to safely model multiple infectious cycles under biosafety level 2 conditions, relying exclusively on Ebola virus components.

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Hoenen, T., Watt, A., Mora, A., Feldmann, H. Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes. J. Vis. Exp. (91), e52381, doi:10.3791/52381 (2014).

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