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线粒体的制备及透气性评估，从骨骼肌组织通过穿刺活检获得的分离
JoVE Journal
医学
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JoVE Journal 医学
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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线粒体的制备及透气性评估，从骨骼肌组织通过穿刺活检获得的分离

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11:27 min

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February 07, 2015

DOI:

10.3791/52350-v

DOI:
10.3791/52350-v

•

11:27 min
February 07, 2015

•

7 Views
, , , , ,
1Department of Internal Medicine, Section on Gerontology and Geriatric Medicine,Wake Forest School of Medicine, 2Seahorse Biosciences

概要

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方法活检股外侧 ，准备纯化线粒体和透气性分析描述。使用小肌肉体积使得该技术适用于临床研究应用。

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Cite this Article

Bharadwaj, M. S., Tyrrell, D. J., Lyles, M. F., Demons, J. L., Rogers, G. W., Molina, A. J. A. Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy. J. Vis. Exp. (96), e52350, doi:10.3791/52350 (2015).

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