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Électrophysiologie en temps réel: en utilisant des protocoles en boucle fermée à la sonde neuronaux Dynamics et au-delà
JoVE Journal
神経科学
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JoVE Journal 神経科学
Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond

Électrophysiologie en temps réel: en utilisant des protocoles en boucle fermée à la sonde neuronaux Dynamics et au-delà

DOI:

08:08 min

June 24, 2015

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  • 00:05標題
  • 01:43Brain Slice Preparation
  • 02:49Patch-clamp Recordings from Layer 5 Pyramidal Neurons
  • 05:35Injection of Conductance Through Simulated Synapses and Simulation of In Vivo-like Background Activity
  • 06:47Results: Recreation of In Vivo-like Activity Using Dynamic Clamp
  • 07:34Conclusion

概要

自動翻訳

Closed-loop protocols are becoming increasingly widespread in modern day electrophysiology. We present a simple, versatile and inexpensive way to perform complex electrophysiological protocols in cortical pyramidal neurons in vitro, using a desktop computer and a digital acquisition board.

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