Closed-loop protocols are becoming increasingly widespread in modern day electrophysiology. We present a simple, versatile and inexpensive way to perform complex electrophysiological protocols in cortical pyramidal neurons in vitro, using a desktop computer and a digital acquisition board.
Linaro, D., Couto, J., Giugliano, M. Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond. J. Vis. Exp. (100), e52320, doi:10.3791/52320 (2015).