The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using <em>Phaseolus vulgaris</em> as an Example

Brendan M. O'Leary; Arantza Rico; Sarah McCraw; Helen N. Fones; Gail M. Preston

doi:10.3791/52113

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The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

JoVE Journal
生物学

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JoVE Journal 生物学

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

35,996 Views

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10:26 min

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December 19, 2014

DOI:

10.3791/52113-v

DOI:

10.3791/52113-v

•

10:26 min

December 19, 2014

•

44 Views

Brendan M. O’Leary¹, Arantza Rico², Sarah McCraw¹, Helen N. Fones³, Gail M. Preston¹

¹Department of Plant Sciences,University of Oxford, ²School of Education of Vitoria-Gasteiz,University of the Basque Country (UPV/EHU), ³Biosciences,University of Exeter

概要

This protocol details the optimized extraction of apoplast washing fluid from plant leaves, using French bean plants (Phaseolus vulgaris) as a model example.

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The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

概要

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