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The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
JoVE 杂志
生物学
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JoVE 杂志 生物学
The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

DOI:
10.3791/52113-v

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10:26 min

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December 19, 2014

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, , , ,
1Department of Plant Sciences,University of Oxford, 2School of Education of Vitoria-Gasteiz,University of the Basque Country (UPV/EHU), 3Biosciences,University of Exeter

Chapters

  • 00:05Title
  • 01:28Leaf Infiltration Using a 60 ml Syringe
  • 03:51Leaf Infiltration by Vacuum Flask
  • 05:13Recovery of Apoplastic Washing Fluid by Centrifugation
  • 06:45Calculation of the Apoplast Dilution Factor
  • 09:02Results: An Example of Metabolite Analysis Made Possible by Apoplast Washing Fluid Extraction
  • 09:45Conclusion

Summary

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This protocol details the optimized extraction of apoplast washing fluid from plant leaves, using French bean plants (Phaseolus vulgaris) as a model example.

Tags

ApoplastApoplastic FluidApoplast Washing FluidInfiltration-centrifugationPlant LeavesPhaseolus VulgarisCell WallExtracellular CompartmentPlant-endophyte InteractionsPlant Defense Responses

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