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Utilisation de l'Essai de superposition pour mesurer qualitativement la production bactérienne et la sensibilité à pneumocoque bactériocines
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JoVE Journal 免疫学と感染
Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins
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Utilisation de l'Essai de superposition pour mesurer qualitativement la production bactérienne et la sensibilité à pneumocoque bactériocines

13,216 Views

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06:05 min

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September 30, 2014

DOI:

10.3791/51876-v

DOI:
10.3791/51876-v

•

06:05 min
September 30, 2014

•

4 Views
,
1Microbiology and Immunology,University of Michigan, 2Pediatrics and Communicable Diseases,University of Michigan

概要

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Competition in Streptococcus pneumoniae is mediated by bacteriocins, small antimicrobial peptides with inhibitory activity towards pneumococcus and other related species.  Here we describe an optimized bacterial overlay assay that allows for the characterization of bacteriocin activity and inhibitory spectrum, bacteriocin-specific immunity, and detection of secreted quorum sensing peptides.

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Maricic, N., Dawid, S. Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins. J. Vis. Exp. (91), e51876, doi:10.3791/51876 (2014).

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