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Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins
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JoVE 杂志 免疫与感染
Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

DOI:
10.3791/51876-v

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06:05 min

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September 30, 2014

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1Microbiology and Immunology,University of Michigan, 2Pediatrics and Communicable Diseases,University of Michigan

Chapters

  • 00:05Title
  • 01:07Preparation of the Producer Strain
  • 02:13Overlay Assay
  • 04:25Results: Representative Overlay Outcomes
  • 05:33Conclusion

Summary

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Competition in Streptococcus pneumoniae is mediated by bacteriocins, small antimicrobial peptides with inhibitory activity towards pneumococcus and other related species.  Here we describe an optimized bacterial overlay assay that allows for the characterization of bacteriocin activity and inhibitory spectrum, bacteriocin-specific immunity, and detection of secreted quorum sensing peptides.

Tags

Overlay AssayBacterial ProductionPneumococcal BacteriocinsStreptococcus PneumoniaeBacteriocin-like Peptide (blp) LocusBacteriocin Immunity Region (BIR)Bacteriocin-mediated CompetitionQuorum Sensing PeptidesLacZ-reporter Strains

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